Mass Spectrometry-based Peptidomics for the Discovery of Antimicrobial Peptides
نویسندگان
چکیده
Most antimicrobial peptides (AMPs) are cationic molecules with a high content of basic amino acids. They often undergo C-terminal amidation that may be related to their antimicrobial activity. Here we describe a mass spectromety (MS)-based approach for identifying novel AMPs. MS is used to profile peptides secreted by endocrine cells. Peptide sequences characteristic of AMPs are selected to test their antimicrobial activity. Our ‘sequence first approach’ has revealed two novel AMPs whose activity against certain microbes are comparable to that of cathelicidin (CATH) or β-defensin-2 (BD2). The antimicrobial activity and natural occurrence of the peptides, designated antimicrobial peptide derived from insulin-like growth factor-binding protein-5 (AMP-IBP5) and VGF [554-577]-NH2, are described in this review.
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